human granzyme b duoset elisa Search Results


human granzyme b  (Bio-Techne corporation)
96
Bio-Techne corporation human granzyme b
(A) GSEA enrichment plot for regulations of T-cell functions and tolerance induction in monocytes following treatment with ATG. Green lines depict running enrichment score, black vertical lines indicate genes responsible for enrichment and position in ranked list of DEG. (B) Violin plots of core enrichment genes CD274, IDO1, IRF1, HLA-A and HLA-E in monocytes isotype versus monocytes ATG. Expression levels are indicated by violin plot height while width represents proportion of positive cells. Crossbars mark mean expression. **** indicate p-value < 0.0001. (C) Representative histogram of proliferative capacity of activated CD8 + T cells co-cultured with monocytes (black), PDL-1 + monocytes (green) and durvalumab pre-treated PDL1 + monocytes (blue). Non-proliferating (furthest right) and proliferating cell populations are reflected by intensity of cell proliferation dye staining. Dashed red line separates cells with 3 or less divisions from cells with 4 or more divisions. (D) Bar graph depicting mean percentages of CD8 + T cells with 3 or less proliferations and 4 or more proliferations at day 5. N = 4 donors per group. *** p-value = 0.0009; ** p-value = 0.0034. One-way ANOVA with Dennett’s multiple comparison was used to determine statistically significant differences. (E) Concentration of <t>granzyme</t> <t>B</t> in conditioned medium of CD8 + T cells co-cultured with monocytes assessed by ELISA. ** p-value < 0.01.
Human Granzyme B, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human granzyme b/product/Bio-Techne corporation
Average 96 stars, based on 1 article reviews
human granzyme b - by Bioz Stars, 2026-03
96/100 stars
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human granzyme b duoset  (R&D Systems)
94
R&D Systems human granzyme b duoset
Protein concentrations of a) <t>granzyme</t> <t>B</t> and b) perforin were measured by ELISA in concentrated bronchoalveolar lavage (BAL) fluid. Dot plots show the distribution of detected levels of granzyme B and perforin, with boxes demonstrating interquartile range, horizontal line showing the median, and whiskers showing the 95% confidence interval. The dotted line represents a) the lowest level of quantitation assay range for granzyme B and b) the limit of detection for perforin. Analysis with Mann–Whitney U-test. BPD: bronchopulmonary dysplasia.
Human Granzyme B Duoset, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human granzyme b duoset/product/R&D Systems
Average 94 stars, based on 1 article reviews
human granzyme b duoset - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


(A) GSEA enrichment plot for regulations of T-cell functions and tolerance induction in monocytes following treatment with ATG. Green lines depict running enrichment score, black vertical lines indicate genes responsible for enrichment and position in ranked list of DEG. (B) Violin plots of core enrichment genes CD274, IDO1, IRF1, HLA-A and HLA-E in monocytes isotype versus monocytes ATG. Expression levels are indicated by violin plot height while width represents proportion of positive cells. Crossbars mark mean expression. **** indicate p-value < 0.0001. (C) Representative histogram of proliferative capacity of activated CD8 + T cells co-cultured with monocytes (black), PDL-1 + monocytes (green) and durvalumab pre-treated PDL1 + monocytes (blue). Non-proliferating (furthest right) and proliferating cell populations are reflected by intensity of cell proliferation dye staining. Dashed red line separates cells with 3 or less divisions from cells with 4 or more divisions. (D) Bar graph depicting mean percentages of CD8 + T cells with 3 or less proliferations and 4 or more proliferations at day 5. N = 4 donors per group. *** p-value = 0.0009; ** p-value = 0.0034. One-way ANOVA with Dennett’s multiple comparison was used to determine statistically significant differences. (E) Concentration of granzyme B in conditioned medium of CD8 + T cells co-cultured with monocytes assessed by ELISA. ** p-value < 0.01.

Journal: bioRxiv

Article Title: Antithymocyte globulin inhibits CD8 + T cell effector functions via the paracrine induction of PDL-1 on monocytes

doi: 10.1101/2022.07.26.501584

Figure Lengend Snippet: (A) GSEA enrichment plot for regulations of T-cell functions and tolerance induction in monocytes following treatment with ATG. Green lines depict running enrichment score, black vertical lines indicate genes responsible for enrichment and position in ranked list of DEG. (B) Violin plots of core enrichment genes CD274, IDO1, IRF1, HLA-A and HLA-E in monocytes isotype versus monocytes ATG. Expression levels are indicated by violin plot height while width represents proportion of positive cells. Crossbars mark mean expression. **** indicate p-value < 0.0001. (C) Representative histogram of proliferative capacity of activated CD8 + T cells co-cultured with monocytes (black), PDL-1 + monocytes (green) and durvalumab pre-treated PDL1 + monocytes (blue). Non-proliferating (furthest right) and proliferating cell populations are reflected by intensity of cell proliferation dye staining. Dashed red line separates cells with 3 or less divisions from cells with 4 or more divisions. (D) Bar graph depicting mean percentages of CD8 + T cells with 3 or less proliferations and 4 or more proliferations at day 5. N = 4 donors per group. *** p-value = 0.0009; ** p-value = 0.0034. One-way ANOVA with Dennett’s multiple comparison was used to determine statistically significant differences. (E) Concentration of granzyme B in conditioned medium of CD8 + T cells co-cultured with monocytes assessed by ELISA. ** p-value < 0.01.

Article Snippet: In addition, we measured IFN-γ in supernatants of ATG-treated whole blood, purified PBMCs and purified CD8 + T cells as well as human granzyme B in co-cultures via ELISA (Bio-techne, R&D, Minneapolis, Minnesota, USA).

Techniques: Expressing, Cell Culture, Staining, Comparison, Concentration Assay, Enzyme-linked Immunosorbent Assay

Protein concentrations of a) granzyme B and b) perforin were measured by ELISA in concentrated bronchoalveolar lavage (BAL) fluid. Dot plots show the distribution of detected levels of granzyme B and perforin, with boxes demonstrating interquartile range, horizontal line showing the median, and whiskers showing the 95% confidence interval. The dotted line represents a) the lowest level of quantitation assay range for granzyme B and b) the limit of detection for perforin. Analysis with Mann–Whitney U-test. BPD: bronchopulmonary dysplasia.

Journal: The European Respiratory Journal

Article Title: Increased cytotoxic T-cells in the airways of adults with former bronchopulmonary dysplasia

doi: 10.1183/13993003.02531-2021

Figure Lengend Snippet: Protein concentrations of a) granzyme B and b) perforin were measured by ELISA in concentrated bronchoalveolar lavage (BAL) fluid. Dot plots show the distribution of detected levels of granzyme B and perforin, with boxes demonstrating interquartile range, horizontal line showing the median, and whiskers showing the 95% confidence interval. The dotted line represents a) the lowest level of quantitation assay range for granzyme B and b) the limit of detection for perforin. Analysis with Mann–Whitney U-test. BPD: bronchopulmonary dysplasia.

Article Snippet: Protein concentrations of Granzyme B and perforin in BAL fluid were quantified using commercial ELISA kits, including Human Granzyme B DuoSet (R&D Systems) and Human Perforin ELISA Set (Diaclone), respectively, following the manufacturer's protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitation Assay, MANN-WHITNEY